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Adv J Emerg Med

Tyrosinase (EC 1.14.18.1), also termed polyphenol oxidase (PPO)a copper-containing metalloprotein is ample in bacteria, fungi, mammals, and plants 3,4 , and their active sites are exceedingly conserved between the diverse species 5,6,7,8

PMID: 17 LiJ.FuN.RenL.LuoY

The QuickChange Site-Directed Mutagenesis Kit (Agilent Technologies, Inc., CA, United States) was used to develop point mutations

Briefly, TBS-soluble fractions were chromatographically separated using a Bio SEC-3 column (4.6 300 mm, 3 m, 5190 2508, Agilent), operated at 25 C

The fixative was discarded followed by a new fixative for electron microscopy, and the cells were observed under TEM (Hitachi)