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The column was pre-heated and sustained at 55C during separation and mobile phase A consisted of 5% (v/v) eluent A (AccQ-Tag Ultra, Waters, Milford, MA, USA) in UPLC grade water, while mobile phase B comprise 100% eluent B (AccQ-Tag Ultra, Waters, Milford, MA, USA)

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What You Should NOT Eat When Trying to Conceive: Bad Food Choices Caffeine Limiting caffeine may be difficult for many people, but your consumption is crucial to be conscious of when trying to get pregnant