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Like with methacholine, the extra methyl side group in bethanechol results in selectivity for muscarinic vs nicotinic receptors, and contributes to resistance to metabolism by cholinesterases

The most prominent change was the rise of a sharp peak at 1045 cm 1 that was assigned to the symmetric S = O stretching mode ((S = O))

Basrah Journal of Veterinary Research

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Ohtake Y, Watanabe K, Tezuka H, Ogata T, Yabuuchi S, Murata K, et al

The thermal cycles conditions applied for each gene were: for NF-B included preliminary denaturation at 95 C for 10 min, followed by 36 cycles of denaturation at 98 C for 30 s, annealing at 60 C for 60 s, and elongation at 72 C for 15 s, followed by a final elongation step at 72 C for 5 min