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Am J Pathol169, 1238250

The samples were then quickly frozen and thawed in liquid nitrogen for 3 cycles, centrifuged at 12,000 rpm for 5 min at 4 C and the supernatant was collected

We suggest using 2-3 times weekly rather than daily

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Targeting neutrophilic inflammation in severe neutrophilic asthma: can we target the disease-relevant neutrophil phenotype