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10.1093/ajcn/nqab193

In a 96 well clear plates, three wells were loaded with 120 L assay buffer (provided) and 20 L GSSG (provided) as background control

The condition is present at birth, but it doesnt manifest until several years into life, and symptoms can take years or decades to progress from mild to severe

doi: 10.1016/j.redox.2016.10.001 28 DuK.RamachandranA.WeemhoffJ

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Using smaller injection volumes (0.20.5 mL) and allowing refrigerated compounds to reach room temperature before injection also minimises tissue irritation