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599 Additionally, Ndfip1 prevents increased expression of ACSL4, significantly improves motor dysfunction, and antagonizes the loss of dopaminergic neurons by inhibiting ferroptosis, 599 indicating that Ndfip1 may be a promising target for iron-related diseases, including PD

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RNA extraction, cDNA synthesis and real-time quantitative PCR (RT-qPCR) Total RNA was isolated from cells using a TRI Reagent kit (Sigma) according to the manufacturers instructions

In the hypoxic microenvironment, AKT and mTOR are located upstream of HIF-1 (156)