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This approach was chosen to streamline the extensive data processing required for hundreds of sample injections, as the well-defined, predictable in vitro cell culture matrix, combined with precise retention time matching, provided sufficient confidence for analyte identification

Docosahexaenoic acid and cognition throughout the lifespan

Western blot (WB) analysis For WBs, cells were extracted in RIPA buffer (Sigma-Aldrich, USA), separated by 10% SDS-PAGE, and transferred onto a polyvinylidene difluoride membrane (Millipore, USA)

The inclusion criteria were (1) research/review articles on sources and generation of reactive free radicals, (2) research/review articles with experimental data on ROS-associated diseases, (3) research/review articles with experimental data on antioxidant activity, (4) research/review articles exploring the mode of action of synthetic as well as natural antioxidants

This includes honoraria, expert testimony, employment, ownership of stocks or options, patents or grants received or pending royalties

doi: 10.1016/j.envexpbot.2009.05.012 106 RehmanM