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Blood was collected in EDTA tubes and centrifuged at 1,600 g for 16 min at room temperature to separate the plasma, which was aspirated and stored at 80 C

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storage at ambient temperature in darkness is recommended Precautions & Notes Stability may be affected by light, heat, and extreme pH Experimental outcomes depend on concentration, model system, and formulation Appropriate controls are recommended for enzymatic and cellular assays Intended strictly for laboratory research use

Therefore, the insights from this study are expected to guide the formulation of future research aimed at developing optimal clinical interventions to improve the prognosis and quality of life for patients with CVD