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2445-C ANTI-INFLAMMATORY AND ANTI-FIBROTIC EFFECTS BY SIMULTANEOUS ACTIVATION OF GLUCAGON, GIP, AND GLP-1 OF EFOCIPEGTRUTIDE (HM15211) IN THIOACETAMIDE-INDUCED MOUSE MODEL OF LIVER INJURY AND FIBROSIS Jung Kuk Kim, Yohan Kim, Hyunjoo Kwon, Eun Jin Park, Jeong A Kim, Sang Hyun Park, Sungmin Bae, Daejin Kim, Sang Hyun Lee and In Young Choi, Hanmi Pharm

Methods: Male C57BL/6 mice were fed either a low fat (CD) or high fat diet (HFD,42%) for 10 weeks

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Curcumin has antioxidant and anti-inflammatory properties

The membrane was first blocked in 5% (wt./vol.) skim milk powder (Lab M Limited, Heywood, UK) in tris-buffered saline with 0.1% tween20 (TBST) for 1 h at RT, then was probed with the primary antibody (concentrations and manufacturers in Supplementary Table 4) diluted in TBST with 5% BSA for 16 h at 4 C on a rocker

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