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Since bacteria exhibit different modes of response to different antibiotic concentrations,[] we used moxifloxacin at MBC levels to maintain the antibiotic concentration always high, with negligible changes in its concentration during the exposure, reported earlier.[] We did not study antibiotic response at single-cell level as antibiotic-exposed single cells in isolation respond differently to antibiotics, unlike the single cells in a population, depending on the antibiotic concentration and exposure duration.[] Formation of three reactive oxygen species in the moxifloxacin-exposed Mycobacterium smegmatis cultures The genetic resisters to moxifloxacin were formed from the cells in the ASP containing the three ROS and Fe (II) at significantly elevated levels

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Fish will be fed based on Tilapia Feeding Guidelines (Cargill, Franklinton, Louisiana) and grown for 100 days

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Without lab equipment at our disposal, we tried to be as fair as possible and chose eight players from the team who all considered themselves to have a similar drinking tolerance