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The expression levels were normalized to the expression of the housekeeping gene RSP-9 (40S ribosomal protein S9) and then quantified using the 2 Ct method according to Livak and Smittgen 62 using StepOne v2.3 software (Applied Biosystems)

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Bacterial contamination risk increases with each needle insertion, and the preservatives in compounded formulations may not provide the same long-term antimicrobial protection as brand-name products