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Sequences of gene-specific primers were as follows: Actin: [forward] 5-ATTGGCAATGAGCGGTTC-3, [reverse] 5-CGTGGATGCCACAGGACT-3, TRIP12: [forward] 5-TTCAGATTGGTGGACCTTCC-3, [reverse] 5-GGCTACAACTGGGTCGATGT-3, OTUD5: [forward] 5-GAGCTGCATGCTGAATTGGG-3, [reverse] 5-AGTCATTCAGACTGGCGCAC-3, IL-6: [forward] 5-TACCCCCAAGGAGAAGATTCC-3 [reverse] 5-TTTTCTGCCAGTGCCTCTTT-3, ADAM8: [forward] 5-GAGGGTGAGCTACGTCCTTG-3, [reverse] 5-CAGCCGTATAGGTCTCTGTGT-3, IRAK2: [forward] 5-GAAATCAGGTGTCCCATTCCAG-3, [reverse] 5-TGGGGAGGTCGCTTCTCAA-3, IL-11: [forward] 5-CTGGGCTAGGGCATGAACTG-3, [reverse] 5-CTGGGACTCCAAGTGCAAGA-3, TBP: [forward] 5-GAGCCAAGAGTGAAGAACAGTC-3, [reverse] 5-GCTCCCCACCATATTCTGAATCT-3

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Inositol phosphate accumulation assay IP1 production was measured using the IP-One HTRF kit (Cisbio, 621PAPEJ) 41

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Supplements and fortified foods contain B12 in its free form, so they may be more easily absorbed." A compromised digestive system with too low of stomach acid or low intrinsic factor greatly increases the risk of low B12 and other nutrients

It has been studied for its potential anti-aging and skin health benefits, as well as wound healing properties