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We used an Ultimate 3000 HPLC system (Dionex) with a Waters Acquity CSH C18 1.7 m 1 150 mm column on operating at a flow rate of 30 l/min with two buffer lines as previously described 23

Since GSH is one of the major antioxidants of the airways, it can be hypothesized that GSTO may participate in the maintenance of GSH not only intracellularly, but also in the extracellular space and this may be modulated by oxidative stress

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