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After 48 h, biofilms were washed once with 1 PBS followed by treatment with different concentrations of 0, 5, 10, 25, and 50 M H 2 O 2 (prepared by diluting an aliquot of 30% (v/v) H 2 O 2 in 1 PBS) for 24 h, at 37C and shaking at 150 rpm

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Livers were homogenized in 10mL of cold 0.1% IGEPAL and spleens were homogenized in 5mL using a Tissue Tearor Model 985370 (Biospec Products) at 10,000 RPM for 5 seconds/organ

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GR activity was measured according to Halliwell & Foyer 55 based on the rate of decrease in the absorbance of NADPH at 340 nm

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coli , this system includes the disulfide isomerase DsbC/G and the reductive power from DsbD, which itself is coupled to the cytoplasmic NADPH pool through Thioredoxin A (TrxA) (Andersen et al., 1997

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Levodopa substitution is still the most effective approach to treat PD motor symptoms, but standard oral drug delivery is associated with plasma level oscillations that give rise to the development of motor fluctuations and dyskinesias

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