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For example, the main clinical manifestation of OSF is a burning sensation and intolerance to spices, and as the disease progresses, patients experience reduction in mouth opening, which not only causes pain, poor oral hygiene and impaired chewing and swallowing, but also affects patients speaking and appearance, causing psychological issues and limitations in social activities (Peng et al., 2020)

Muhammad N, Hakim Quraishi U, Chaudhary HJ, Munis MFH

Am J Physiol Regul Integr Compr Physiol 310:R1R14

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Tissues were homogenized and lysed in T-per Tissue protein extraction reagent (78510, Thermo Fisher Scientific) using a bead homogenizer for 3 min at maximum speed (Qiagen, TissueLyser II) for total tissue protein