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For this experiment, the temperature was maintained at 60 C, as OsDHAR and catalase (CAT) aggregate at this temperature over time (Fig

Mast cells and aging

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Thereafter, anhydrous sodium sulfate was added to remove moisture from the product which was then filtered and distilled under reduced pressure to remove ethyl acetate, followed by fractional distillation under reduced pressure (93-95 C/5 mmHg) to obtain 40.4 g (yield: 80 %) of ethyl 4-chloro-3-hydroxybutyrate

Topalova-Dimitrova A, Dimitrov IV, Nikolov R

Tumor-suppressor p53: implications for tumor development and prognosis