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ZR-75-1 and T47D cells were cultured in DMEM with 10% FBS supplemented with 100 units/mL penicillin and 100 g/mL streptomycin

Table 4 Characteristics of preclinical models for NP and NPP and their corresponding potential targets

Kanwal S, Yousaf Q, Jamil A, Arshad W, Qadir M, Tahir MU, Rahim F, Ahmed U, Habib U

229,230 Neuroendocrine-immune axis Microglia secrete chemokines that disrupt the integrity of the BBB and increase the ability of immune cells to enter the brain parenchyma

By week 32, all the phenotypes advanced to a more severe stage