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All cells except for hNF-1 were grown and maintained as monolayer cultures at 37 C in 5% CO 2 in a humidified atmosphere, using DMEM (high glucose with l-glutamine and phenol red, Fujifilm Wako Pure chemical corporation), supplemented with penicillinstreptomycin solution (100) (Fujifilm Wako Pure Chemical Corporation), and 10 vol% FBS (Sigma-Aldrich Co., Llc.)

Adoptive transfer of vaccine-induced peripheral blood mononuclear cells to patients with metastatic melanoma following lymphodepletion

10.1371/journal.pone.0223435 40 LeeJ

[DOI] [PMC free article] [PubMed] [Google Scholar] 78.Aparicio S, et al

A liter of IV fluids with electrolytes enters your bloodstream directly, expanding blood volume within minutes

La FaziaVMPierucciNMohantySGianniCDella RoccaDGCompagnucciPet alCatheter ablation approach and outcome in HIV+ patients with recurrent atrial fibrillation