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cDNA was synthesized using a reverse transcription system kit according to the manufacturers instructions (PrimeScript RT reagent Kit with gDNA Eraser, RR047A, TaKaRa, Japan)

Previous attempts to overcome these problems have either been unsuccessful and/or not feasible

[1] It often forms nodules, and can be associated with fibrosis and adhesions

Ethics declarations Ethics approval and consent to participate Institutional review board (IRB) approval covering patient data contained in the OPERA database was received from Advarra IRB

The microscopy images showed cell rupture and lysis (H&E stain), indicative of signs of cell death (TUNEL stain) in the tumor tissue (Fig

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