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FC experiment of HepG2 using 66323-2-Ig 1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Glutamine Synthetase (66323-2-Ig, Clone:1B6G4) and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue)

Model building For the short (10aa) ARF1-linker RNC with NMT1 and NAC, the overall ribosome model from PDB: 6Y2L 57 and proteins eS12 and eS31 from PDB: 8GLP 58 were rigid body docked in the cryo-EM density