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Einige Lebensmittel enthalten natrlicherweise Glutathion, das den Glutathion-Stoffwechsel im Krper untersttzen kann

Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected

The proteome concentration of the tissue lysates was determined using the DC protein assay (Bio-Rad), normalized to 2 mg/mL, and then 100 L of each sample was transferred to a LoBind Eppendorf tube containing 48 mg of urea

doi: 10.1016/j.wneu.2021.05.098 199 LiuTZhuCChenXGuanGZouCShenSet al

The analysis of the optimized structures of the MichaelisMenten complex indicate that the polar residues at position 13 (Cys or Ser) stabilize the deprotonated GS , which in turn acts as the catalytic Brnsted base and abstracts the proton at position C2 of substrate 7 (Extended Data Fig

e , Change in fat mass