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Whole blood histamine: This test measures histamine levels in whole blood, which can provide additional insights into histamine metabolism

The sections were then incubated with primary antibodies against occludin (Abcam, dilution 1:100) and ZO-1 (ThermoFisher, dilution 1:100), diluted in PBS containing 0.2% BSA and 0.1% saponin at 4 C overnight, washed, and incubated for 1 h with Alexa Fluor 647 donkey anti-rabbit IgG secondary antibody (Invitrogen, dilution 1:400)

Two ways to prevent contamination by leakage: Holding tanks must be verified for resistance to chemical sanitizers

Zhang H, Yu F, Tian Z, Jia D

Attenuation of CD4(+)CD25(+) regulatory T cells in the tumor microenvironment by metformin, a Type 2 diabetes drug