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The column temperature was 30 C, and the mobile phase was a mixture of A [50 mM phosphate buffer (pH 3.0) containing 10 mM sodium 1-heptanesulfonate] and B (methanol) with an A/B ratio of 95/5 at a flow rate of 1 mL/min

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Then the cells were centrifuged and washed once with 1 ml of 0.1 M borax (Sigma-Aldrich, 71997), followed by another wash with 1% BSA in PBS

58 (2), M17680