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Stroes ES, van Faassen EE, Yo M, Martasek P, Boer P, Govers R, Rabelink TJ (2000) Folic acid reverts dysfunction of endothelial nitric oxide synthase

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Lesion area measurements were performed in 8 equidistant sections obtained from the aortic root of each animal (Figure 3B)

Almost no red fluorescence signal was detected when CT26 cells were pre-incubated in 4C condition, manifesting that CT26 cells internalized MCAP NPs in an energy-dependent mechanism (Figures 3A,C and Supplementary Figure S11C)

Next, cells were observed by fluorescence microscope for visualization and were analyzed by flow cytometry for quantification