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Cells were then stained with 0.5% (vol/vol) crystal violet solution (Sigma, HT90132-1L) at room temperature for 10 minutes, on a rotating shaker

Free radicals, owing to their instability and consequent erratic behavior, have the potential to cause cell damage that would be virtually unlimited without antioxidants to hold their numbers in check and glutathione, which is the only antioxidant that is known to occur naturally within the cells is the master antioxidant. Although it was first identified in 1888, it was not until almost a century later that scientists began to undertake through investigations into the role that it plays in human metabolism

doi: 10.1097/SHK.0000000000000044

22 Prevalence and Geographic Distribution Deficiency of G6PD is the most common metabolic disorder of red blood cells and has been estimated to affect over 400 million people worldwide

Matson et al., 2011)