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Caraballo JC, Yshii C, Westphal W, Moninger T, Comellas AP

The advanced glycation end products (AGE) content in the plasma membrane of epithelial cells is directly increased by the conversion of the superoxide anion to hydrogen peroxide by SOD3, as seen in ( Figure 4 )

Cells were plated on 48-well plates, coated with anti-CD3 (1 g ml 1 ) and anti-CD28 (10 g ml 1 ) antibodies, in RPMI medium supplemented with 10% FCS, penicillin, streptomycin, HEPES, glutamine, pyruvate, nonessential amino acids and -mercaptoethanol diluted with PBS (1:1) in the presence of IL-12 (3 ng ml 1 , Peprotech) (T H 1 conditions)

Experimental observations are intended exclusively for scientific exploration and hypothesis development within regulated research environments

Notch1 and Notch2 are highly expressed in glioma cell lines as well as primary human gliomas [11, 41]