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Cellular experiments further confirmed that silencing SIRT3 expression significantly promoted hypoxia- and doxorubicin (DOX)-induced cardiomyocyte apoptosis and lactate dehydrogenase (LDH) release, upregulated SOD2 expression and increased ROS release, inhibited Opa1, PINK1, and Parkin protein expression, and significantly increased Drp1, LC3- II, and p62 expression, suggesting that short-term aerobic exercise mediates SIRT3 protection against aging myocardial injury (Zhao D

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Mice were anesthetized with 1.5% (v/v) isoflurane and anchored in the apparatus

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