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Suo et al., 2020) inducing channel opening (Hinman et al., 2006

After washing with five column volumes of equilibration buffer, elution was performed applying a gradient over 10 column up to 500 mM imidazole

Determinants of the cytosolic turnover of mitochondrial intermembrane space proteins

M., King S

The 72-hour minimum is a safety guideline, not a suggestion