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The lysis buffer for western blotting was obtained from Santa Cruz Biotechnology Inc

doi: 10.1074/jbc.273.25.15514 45 SerenSDerianLKelesIGuillonALesnerAGonzalezLet al

In patients with end-stage liver disease, this treatment not only is less effective but can also be deleterious, and therefore patients need to be selected carefully [82]

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Most research protocols observe significant GH elevation for 60-120 minutes post-administration, with gradual decline thereafter as the body metabolises the compound