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Methods have been developed to systematically screen phosphosite function by mutagenesis in yeast [111], and the creation of similar methods for mammalian systems would greatly aid this task

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10.1074/jbc.M111.253039 Summary Keywords stable gastric pentadecapeptide BPC 157, fistula, bleeding disorder, concept practical applicability, wound healing Citation Seiwerth S, Milavic M, Vukojevic J, Gojkovic S, Krezic I, Vuletic LB, Pavlov KH, Petrovic A, Sikiric S, Vranes H, Prtoric A, Zizek H, Durasin T, Dobric I, Staresinic M, Strbe S, Knezevic M, Sola M, Kokot A, Sever M, Lovric E, Skrtic A, Blagaic AB and Sikiric P (2021) Stable Gastric Pentadecapeptide BPC 157 and Wound Healing

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The R12 reporter assay showed that the arrest products were gently reduced in both single deletion mutants, ubp2 and ubp3 , and synergistically reduced in the double deletion mutants, ubp2ubp3 (Fig