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Quantification of H 2 S and GSSH from oxidatively stressed mitochondria Rat heart mitochondria (1 mg protein/mL) were suspended in KCl buffer (pH 7.2) containing 1 mM EGTA and treated with carrier (KCl buffer), Na 2 S (250 M) or H 2 O 2 (50 M) for 3 min, or H 2 O 2 (50 M) for 3 min followed by and Na 2 S (250 M) for 3 min at RT