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31 Briefly, to 1 g DNA, RNase A (Sigma, St Louis, MO, USA) was added to a final concentration of 0.02 mg ml 1 and incubated at 37 C for 15 min

To investigate inflammation and pyroptosis, immunohistochemistry was employed to assess the expression of key molecules in the penile corpora cavernosa

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Ferroptosis and RF exhibit a mutually reinforcing relationship: in CKD renal tissues, lipid peroxidation, necroinflammation, and iron overload damage parenchymal cells, driving fibrotic accumulation (189)

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