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While there are different models to study glutathione metabolism, in this study we used BSO as it is a specific and potent chemical inhibitor of the rate limiting enzyme GCLC (38)

Sapmaz et al., 2022)

Reading the unique DNA methylation landscape of the brain: non-CpG methylation, hydroxymethylation, and MECP2

Screening by determining total homocysteine (tHcy) in dried blood spots is only occasionally performed given the need for a reduction step prior to LC-MS/MS assay and associated costs [42]

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Chronic alcohol feeding combined with multiple high-dose alcohol gavages can simulate the drinking patterns of most patients with liver disease and are highly similar to the pathogenesis of human ALD