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For vWF assessment, the cells were fixed with 4% paraformaldehyde, washed three times with PBS and incubated in 10% bovine serum albumin for 30 min to block non-specific binding

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Recently, in vitro cell scratch assay to examine the healing effects of peptides and a positive control epidermal growth factor (EGF)

However, individual nucleotides known to be important for GABA and melatonin release [adenosine 5monophosphate (5AMP), guanosine 5-monophosphate (5GMP), uridine 5-monophosphate (5UMP), cytidine 5-monophosphate (5CMP), and inosine 5-monophosphate (5IMP)] [41]

Drugs developed based on this hypothesis, however, have not met with much success in recent times