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Although Compounds 1.62 and 1.64 lost activity for the GPx and/or GR enzymes responsible for the antioxidative effect and regeneration of the thiol group of GSH using other reductant sources, respectively, these compounds were still able to rescue the cell death of UVA irradiated cells as the neutralisation the free radicals produced by UVA irradiation could be achieved by other pathways

10.1021/ci100031x J

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After sonicating (1400 rpm, 20 C, 5 min), the extracts were cleared by centrifugation (17,000 g , 20 C, 10 min), and the lipids in the supernatant were analyzed by LC-MS

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Glutathione degradation is a key determinant of glutathione homeostasis