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The area of the axon (A) and myelin sheath (MS) of sciatic nerves was used to calculate the ratio of axon to myelin sheath area (A/MS)

After 2 days, the medium was replaced with DMEM supplemented with 1 mg ml 1 insulin in 10% FBS and P/S (day 2), and it was changed every 2 days from day 4 to 8

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