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At E20, E24, or E28, pregnant ferrets were anesthetized by intramuscular injection of ketamine (20 mg/kg of body weight, BelaPharm) and xylazine (1 mg/kg, PharmaPartner) and euthanized by intracardiac injection of T61 (0.3 ml/kg of body weight, Intervet), followed by the immediate removal of embryos and dissection of whole heads (E20) or brains (E24, E28) in PBS and immediate fixation

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Identification of new OPA1 cleavage site reveals that short isoforms regulate mitochondrial fusion

As of now, it is available through compounding pharmacies and should be used under the direction of a qualified provider