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For nuclei extraction, the cells were incubated with 0.1% (v/v) digitonin (Promega, USA) for 3 min at 4 C and centrifuged for 10 min at 500 x g at 4 C with 1 mL ATAC-RSB, containing 0.1% (v/v) Tween-20

The specific signal for each sample was calculated after subtracting the background signal obtained for antigen-free wells

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When a connective target suffers severe mechanical trauma, the resulting micro-tears rapidly drop local oxygen tension, creating an ischemic cellular environment

For example, miRNAs generated by Cuscuta campestris (such as miR12463b and miR12495) target the 5 and 3 regions of BIK1 , a critical factor in the Arabidopsis immune signaling pathway

Future Oncol (2020) 16(18):126987