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I infected 9 CC lines with LACV and identified diverse susceptibility phenotypes among the lines

The effectiveness of topical applications has been questioned due to vitamin Cs instability (it reacts with water and degrades)

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To incubate DC2 cells with EV, the above protocol was followed with the exception that at the indicated times culture medium was supplemented with 3.14 10 8 EVs/mL in PBS, or an equal volume of PBS control for 3 days