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Samples were denatured at 95 C for 10 min and separated by 15% SDS-PAGE

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Moreover, replacements of Lys105 resulted in a reciprocal increase of the K m app (GSSCys) values and decrease of the K m app (GSH) values (Fig

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This paper systematically reviews the molecular mechanisms of disulfidptosis, elucidates its regulatory role in male cancer cellsincluding key regulatory genes and therapeutic target potentialand discusses its application value and challenges as a potential therapeutic target based on clinical research

then, hydroxylamine chloride (51 mM) in an alkaline solution (0.5 M sodium carbonate) was added and absorbance was estimated at 560 nm