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After quantification, 1 g of RNA was retro-transcribed by M-MLV reverse transcriptase (Invitrogen, CA, United States) and used in quantitative RT-PCR (qPCR) experiments using Power-up Sybr green PCR master mix (Applied Biosystem, CA, United States) following manufacturer's instructions

The chromosomal localization, GO enrichment annotation, subcellular localization, syntenic relationship, Ka/Ks analysis, and molecular characteristics were accomplished using various bioinformatics tools

Toward Sustainable Scaled-Up Synthesis of Lignin-Derived Carbon Nanoparticles Using a Furnace Aerosol Reactor: Insights from a Life Cycle Assessment