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Abbreviations GSH: Glutathione GR: Glutathione reductase GR1: Chloroplastic GR GR2: Cytosolic GR Fe: Iron References Alfenito M, Souer E, Goodman CD, Buell R, Mol J, Koes R, Walbot V (1998) Functional complementation of anthocyanin sequestration in the vacuole by widely divergent glutathione S-transferases

L-cysteine is the rate-limiting substrate for the synthesis of glutathione

Finally, the stained cells were observed and imaged under a fluorescence microscope and mean immunofluorescence intensity was quantitatively analyzed with ImageJ software