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But they deserve it

Molecular Microbiology, 114(3), 377390

Plate was washed three time and chromogenic substrate 3,3,5,5-tetramethylbenzidine (TMB) which catalyzes by HRP to generate a blue color was added

The mixture was turned into orange red by minimum addition of deionized water recorded with pH 6.2 after 12 h incubation

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