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The supernatant was discarded, and the bacterial pellet was resuspended in PBS and washed twice

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micobiologyOpen 5 , 320 (2016)

CVOs enable a diet induced CNS glutamate (excitatory)/GABA (inhibitory) imbalance to precipitate not only POTS but also symptoms that involve decreased oxytocin, vasopressin (ADH), Corticotropin Releasing Hormone (CRH for ACTH), and Gonadotropin Releasing Hormone (GnRH for LH, FSH) [50,51]