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The glutamate carbon skeleton originates from the tricarboxylic acid (TCA) cycle intermediate -ketoglutarate, while the amino group is derived from nitrogen donors such as the BCAAs

doi: 10.4103/aian.AIAN_770_20 165 RathnayakaNRanathungaANRathnayakaBKularatneSAM

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It plays a critical role across multiple physiological systems: Efficient methylation depends on adequate availability of active folate (5-methyltetrahydrofolate, 5-MTHF) and vitamin B12 (as methylcobalamin) , along with properly functioning enzymes within the one-carbon metabolism cycle