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FlaB was amplified by forward primer (5-TTGCTGATCAAGCTCAATATAACCA-3) and reverse primer (5-GCATCGCTTTCAGGGTCTCAA-3) with a probe quenched with FAM fluorescent dye (5-AGAACAGCTGAAGAGCTTGGAATGCAGCCTGCAAAAATTAACACA-3)

Concurrently, cholesterol released by tumor cells into the TME drives immunosuppressive reprogramming by activating myeloid-derived suppressor cells (334)