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[DOI] [PMC free article] [PubMed] [Google Scholar] 29.Woolf K., Hahn N.L., Christensen M.M., Carlson-Phillips A., Hansen C.M

Available evidence suggests a role for voltage-dependent anion channel (VDAC), located in the OMM, and adenine nucleotide translocase (ANT) across the IMM ( 2 + -induced swelling and MPT, although at a higher threshold, which has been interpreted as evidence against a role for ANT in MPT (Sileikyte et al., 2014)

doi: 10.1038/s41598-017-01732-1 80 ConnellyPJPrenticeNPCouslandGBonhamJ

The plates were then washed twice with ice-cold PBS, scraped, pelleted 250 g at 4 C for 3 min and lysed with RIPA buffer (50 mM Tris-Cl (pH = 8), 150 mM NaCl, 5 mM EDTA, 1 % NP-40, 0.5 % sodium deoxycholate, 0.1% SDS, 1x protease and 1x phosphatase inhibitors)

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However, since the vital role of this phenomenon in other neurological dysfunction such as Parkinsons disease as mentioned above, it is essential to explore the concrete role of ubiquitination by HDACs for epileptic progression