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Note that immunoreactivity was lost after chemical fixation possibly attributed to derivatization of conserved lysine residues in the epitope ( Supplementary Fig

The sample was subsequently centrifuged at 13,000 g for five minutes, after which the supernatant was transferred to an injection vial for GC-MS analysis [23, 24]

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Interventions may include targeted nutritional support, correction of iron and vitamin deficiencies, management of chronic inflammation, and careful monitoring of erythropoietic function