glutathione press Reduced Important Care To Be Taken

doi: 10.3390/antiox11010050 153 KwonD

Razandi M, Pedram A, Jordan VC, Fuqua S, Levin ER

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Following treatment, cells were fixed with 4 % paraformaldehyde (w/v)/4 % sucrose (w/v) solution and stained for alkaline phosphatase activity using a BCIP/NBT kit according to the manufacturers instructions (Vector labs, Burlingame, CA)

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Then, the samples were counterstained with DAPI staining solution for 10 min at room temperature shielded from light, washed three times with PBS (pH 7.4), and sealed with an anti-fluorescence quenching agent