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Glutathione S-transferase (GST) activity was assessed by adding 10 L of the tissue supernatant to a mixture of 10 L of GSH (5 mM) and 10 L of 2,4-dinitrobenzene (CDNB) (1 mM) in 100 L of phosphate buffer, recording at 340 nm for 5 min, and the activity was expressed as moles of CDNB conjugated/min/mg of protein (Al Kury et al., 2019)

Vasconcelos, D

If its not needed, then stop it or reduce the dose

In particular, miR-16 was reported to be downregulated in osteosarcoma cell lines and tissues (77)

Reduce dose by 25-50% and reassess Numbness or tingling in hands: Classic sign of GH-related carpal tunnel from fluid retention

IGF-1 DES has a shorter half-life and more localized effect, which means it carries less systemic risk but more site-specific irritation